abstract：:The requisite endpoint of mammalian oocyte maturation, whether in vivo or in vitro, is a metaphase II oocyte which is able to be fertilized and which can eventually support normal embryonic development. Oocytes which have been matured in vivo basically fulfill these criteria. On the other hand, a completely different ...

journal_title：Molecular human reproduction

authors： Fulka J Jr,First NL,Moor RM

更新日期：1998-01-01 00:00:00

abstract：:We purified by fractionation on 10-40% glycerol gradients, 26S proteasomes from normal human spermatozoa. These proteasomes, which participate in the ATP-dependent degradation of ubiquitinated proteins, share a similar sedimentation coefficient to those purified from other human tissues. Fluorogenic peptide assays rev...

journal_title：Molecular human reproduction

authors： Tipler CP,Hutchon SP,Hendil K,Tanaka K,Fishel S,Mayer RJ

更新日期：1997-12-01 00:00:00

abstract：:Mammalian sperm chromatin is highly condensed, so isolating DNA from such chromatin can be a formidable task. The procedures that produce high quality DNA from somatic cells fail to yield quality sperm DNA. In this study we have modified the previously used guanidinium method to make it simple and efficient in isolati...

journal_title：Molecular human reproduction

authors： Hossain AM,Rizk B,Behzadian A,Thorneycroft IH

更新日期：1997-11-01 00:00:00

abstract：:In the interest of classifying cases of male factor infertility, we have paid particular attention to the sugar ligand binding properties of the human sperm surface and the functional capacity of the acrosome for exocytosis--key parameters for assessing sperm fertilizing ability. Zona recognition and binding involve t...

journal_title：Molecular human reproduction

authors： Benoff S,Hurley IR,Mandel FS,Cooper GW,Hershlag A

更新日期：1997-10-01 00:00:00

abstract：:Nitric oxide (NO) is synthesized from L-arginine by a family of enzymes known as the nitric oxide synthases (NOS). We have recently shown a NOS similar to constitutive brain NOS (bNOS) and endothelial NOS (ecNOS) to be present in spermatozoa. The aim of this study is to investigate NO production by human spermatozoa a...

journal_title：Molecular human reproduction

authors： Donnelly ET,Lewis SE,Thompson W,Chakravarthy U

更新日期：1997-09-01 00:00:00

abstract：:Controlling the sex of offspring by the separation of X and Y chromosome-bearing spermatozoa using flow cytometry has been reported as a clinical technique aiding prevention of X-linked diseases. Although this technique has resulted in several hundred normal births in animals and at least one human birth, there is sti...

journal_title：Molecular human reproduction

authors： Catt SL,Sakkas D,Bizzaro D,Bianchi PG,Maxwell WM,Evans G

更新日期：1997-09-01 00:00:00

abstract：:Several reports demonstrating the presence of messenger RNAs for sperm-specific nucleoproteins, the protamines PRM-1, PRM-2, and the transition protein TP-1 as well as beta-actin, c-MYC, HLA1, and beta 1 integrin have challenged the accepted view of the transcriptional dormance of terminally differentiated spermatozoa...

journal_title：Molecular human reproduction

authors： Miller D

更新日期：1997-08-01 00:00:00

abstract：:G12 monoclonal antibody (mAb), one of a library of constructed mAb directed against human sperm proteins, was found by immunoperoxidase staining to label the post-acrosomal and neck regions of fixed human cauda epididymal and ejaculated spermatozoa. Epithelium and fluid of caput epididymis were strongly labelled while...

journal_title：Molecular human reproduction

authors： Lefèvre A,Martin Ruiz C,Chokomian S,Duquenne C,Finaz C

更新日期：1997-06-01 00:00:00

abstract：:Villous tissues from 30 spontaneous abortions and the same number of artificial abortions were obtained and analysed for the frequency of polyploid cells. Single cell suspensions were made from these tissues without culture and the ploidy of > 100 cells was analysed. Trisomies of chromosomes 17 and 4 have rarely been ...

journal_title：Molecular human reproduction

authors： Horiuchi I,Hashimoto T,Tsuji Y,Shimada H,Furuyama J,Koyama K

更新日期：1997-05-01 00:00:00

abstract：:The Prader-Willi (PWS) and Angelman (AS) syndromes are two clinically distinct syndromes which result from lack of expression of imprinted genes within chromosome 15q11-q13. These two syndromes result from 15q11-q13 deletions, chromosome 15 uniparental disomy (UPD), imprinting centre mutations and, for AS, probable mu...

journal_title：Molecular human reproduction

authors： Glenn CC,Driscoll DJ,Yang TP,Nicholls RD

更新日期：1997-04-01 00:00:00

abstract：:A molecule isolated from the peritoneal fluids of women undergoing laparoscopy for in-vitro fertilization techniques has been chemically characterized and identified as 1-palmitic-3-phosphorylcholine (lysophosphatidylcholine, LPC). This lipid is able, at physiological concentrations, to completely inhibit sperm motili...

journal_title：Molecular human reproduction

authors： Keller F,Togni G,Soldati G,Balmelli T,Medici G,Rose K,Balerna M

更新日期：1997-03-01 00:00:00

abstract：:Patients with rare overgrowth disorders, such as Beck-Wiedemann syndrome and Simpson-Golabi-Behmel syndrome, are predisposed to embryonal tumours, including Wilms' tumour of the kidney. Therefore, these disorders offer a link between hyperplastic growth and cancer. Genetic lesions at chromosome 11p15 have been associa...

journal_title：Molecular human reproduction

authors： Ward A

更新日期：1997-02-01 00:00:00

abstract：:Immunocytochemistry was used to localize endothelial (eNOS) and inducible (iNOS) nitric oxide synthase in human uterine tissues collected at various stages of the menstrual cycle, after exposure to exogenous progestagens, and in early pregnancy. Endothelial NOS-like immunoreactivity was detected in all specimens in en...

journal_title：Molecular human reproduction

authors： Telfer JF,Irvine GA,Kohnen G,Campbell S,Cameron IT

更新日期：1997-01-01 00:00:00

abstract：:Successful intracytoplasmic sperm injection (ICSI) is dependent upon the competence of the oocyte to respond to the injection of a spermatozoon in the presence of calcium. This study determined if oocytes that failed to become fertilized (absence of any pronuclei) failed to undergo cytoplasmic activation, as ascertain...

journal_title：Molecular human reproduction

authors： Gross V,Dubey A,Penzias AS,Layman L,Reindollar R,Ducibella T

更新日期：1996-12-01 00:00:00

abstract：:Prostaglandins (PG) E2 and F2 alpha are produced by the human corpus luteum in significant amounts and they are known to exert local luteotrophic and luteolytic effects respectively. The present study determined how PGE2 and PGF2 alpha are involved in the regulation of inhibin A, a granulosa cell-derived glycoprotein ...

journal_title：Molecular human reproduction

authors： Erämaa M,Ritvos O

更新日期：1996-11-01 00:00:00

abstract：:Preimplantation factor (PIF) is detected in the serum of women shortly after fertilization; its origin, however, has not been established. In this study, the embryonal origin of PIF was investigated and partial characterization of the factor was carried out. Culture media from viable human 2-8-cell stage embryos and m...

journal_title：Molecular human reproduction

authors： Roussev RG,Coulam CB,Kaider BD,Yarkoni M,Leavis PC,Barnea ER

更新日期：1996-11-01 00:00:00

abstract：:This study was undertaken to evaluate the effects of gamma-aminobutyric acid (GABA) and GABAergic agonists and antagonists on sperm kinematic parameters and hyperactivation, evaluated by a computer-assisted semen analysis (CASA) system, and intracellular cAMP content in 22 normozoospermic semen samples. Because of the...

journal_title：Molecular human reproduction

authors： Calogero AE,Hall J,Fishel S,Green S,Hunter A,D'Agata R

更新日期：1996-10-01 00:00:00

abstract：:Glycoforms of recombinant human follicle stimulating hormone (rhFSH) (Org 32489, Puregon) were characterized using concanavalin A lectin affinity chromatography to reveal information about the internal carbohydrate complexity (extent of carbohydrate side-chain branching) of the preparations. The rhFSH glycoforms were ...

journal_title：Molecular human reproduction

authors： Harris SD,Anobile CJ,McLoughlin JD,Mitchell R,Lambert A,Robertson WR

更新日期：1996-10-01 00:00:00

abstract：:A rapid fluorescence in-situ hybridization (FISH) technique was used for direct chromosomal analysis on germ cells from an infertile male with large-headed spermatozoa. The interphase chromosomes were fluorescently-labelled using an extremely bright cyanine dye during a 5-15 min FISH procedure. Germ cells were analyse...

journal_title：Molecular human reproduction

authors： Yurov YB,Saias MJ,Vorsanova SG,Erny R,Soloviev IV,Sharonin VO,Guichaoua MR,Luciani JM

更新日期：1996-09-01 00:00:00

abstract：:Recombinant human ZP3 (rhuZP3) generated by Chinese hamster ovary cells transfected with a plasmid containing human ZP3 cDNA was used to study the acrosome reaction (AR) and intracellular calcium fluxes in capacitated human spermatozoa. Conditioned medium containing rhuZP3 significantly induced the AR (P < or = 0.005)...

journal_title：Molecular human reproduction

authors： Brewis IA,Clayton R,Barratt CL,Hornby DP,Moore HD

更新日期：1996-08-01 00:00:00

abstract：:In order to improve assisted fertilization in humans it is important to elucidate the mechanisms of control of growth and development in the early pre-embryo. Increasing evidence shows that growth factors are of importance for such control mechanisms. As platelet-derived growth factor (PDGF) has been shown to enhance ...

journal_title：Molecular human reproduction

authors： Osterlund C,Wramsby H,Pousette A

更新日期：1996-07-01 00:00:00

abstract：:The involvement of protein kinases in platelet activating factor (PAF)-induced acrosome reaction of human spermatozoa was investigated using specific inhibitors of protein kinase A (PKA), protein kinase C (PKC) and protein tyrosine kinase (PTK). PAF (10(-9)-10(-11) M) treatment of spermatozoa enhanced the acrosome rea...

journal_title：Molecular human reproduction

authors： Sengoku K,Tamate K,Takuma N,Takaoka Y,Yoshida T,Nishiwaki K,Ishikawa M

更新日期：1996-06-01 00:00:00

abstract：:The expression of adhesion molecules and matrix proteins by human spermatozoa as well as their binding to and penetration of zona-free hamster eggs were investigated in 17 patients by means of flow cytometry. Both binding and penetration of hamster oocytes and expression of alpha- and beta-chains of beta 1, beta 3 and...

journal_title：Molecular human reproduction

authors： Henkel R,Schaller J,Glander HJ,Schill WB

更新日期：1996-05-01 00:00:00

abstract：:This study was undertaken to localize ryanodine-sensitive Ca2+ stores in human oocytes and to evaluate their role in the Ca2+ oscillations responsible for oocyte activation at fertilization. The addition of ryanodine provoked a Ca2+ discharge from stores localized throughout the ooplasm with the exception of the corti...

journal_title：Molecular human reproduction

authors： Sousa M,Barros A,Tesarik J

更新日期：1996-04-01 00:00:00

abstract：:We describe a rapid and specific differential amplification system which can detect five of the most common cystic fibrosis mutations from a single cell. In the first round of the polymerase chain reaction (PCR), regions of exons 4, 10 and 11 of the cystic fibrosis transmembrane conductance regulator (CFTR) gene conta...

journal_title：Molecular human reproduction

authors： Scobie G,Woodroffe B,Fishel S,Kalsheker N

更新日期：1996-03-01 00:00:00

abstract：:Individual spermatozoa from the father of two affected infants with osteogenesis imperfecta were separated by dilution and micromanipulation. A segment of the type I collagen gene containing the mutant region was amplified by nested polymerase chain reaction and sequenced. Among 40 individual spermatozoa, 15 specimens...

journal_title：Molecular human reproduction

authors： Iida T,Suzumori K,Ikuta K,Tanemura M,Yagami Y,Okamoto T,Hata A

更新日期：1996-02-01 00:00:00

abstract：:Because of its distinctive ability to act as a mitogen, a mitogen and a morphogen, hepatocyte growth factor/scatter factor (HGF/SF) has all the characteristics of a molecule able to function in regulatory networks of motility, such as the spermatogenic epithelium, and this through binding of its receptor p190MET (C-ME...

journal_title：Molecular human reproduction

authors： Depuydt CE,Zalata A,de Potter CR,van Emmelo J,Comhaire FH

更新日期：1996-01-01 00:00:00

abstract：:An understanding of the relationship between nuclear morphology and DNA function is important in cytology and preimplantation diagnosis. In this study, direct polymerase chain reaction (PCR) amplification was used to diagnose the common delta F508 mutation of cystic fibrosis in 62 biopsied human embryo cells. The nucl...

journal_title：Molecular human reproduction

authors： Cui KH,Matthews CD

更新日期：1996-01-01 00:00:00